Determinants of Mouse Alpha‐Defensin Bactericidal Activity

Absent Bactericidal Activity of Mouse Serum

Mouse splenic peroxidase and its role in bactericidal activity.

Spleen cell suspensions from AKR and CD-1 mice contain peroxidase activity as determined by guaiacol oxidation. This activity is found predominately in the 20,000 x g pellet fraction of spleen cell homogenates. In the presence of H(2)O(2) and chloride ion at acidic pH, splenic peroxidase mediates the oxidation of d- or l-alanine to CO(2), NH(3), and acetaldehyde. The same reaction mixture witho...

Bactericidal Activity

as frequent in those with bactericidal activity (54%) as in those without (56%). The coexistence of bactericidal activity and bacteremia was reproduced and studied in experimentally infected weanling rats. Serum from such rats kills in vitro 95% of conventionally broth-grown bacteria within 10 min, but does not kill organisms obtained from the infected animals. Thus bactericidal activity as con...

Bactericidal Activity of C

Bactericidal antibody is required for direct complement-mediated killing of most pathogenic gram-negative bacteria. Although it has been implicitly assumed that bactericidal antibody functions by increasing complement activation and deposition on the bacterial surface, this assumption may not be valid. We have shown previously (1) that bactericidal IgG increases the bactericidal efficiency of C...

878 Bactericidal Activity Of

Bactericidal antibody is required for direct complement-mediated killing of most pathogenic gram-negative bacteria. Although it has been implicitly assumed that bactericidal antibody functions by increasing complement activation and deposition on the bacterial surface, this assumption may not be valid. We have shown previously (1) that bactericidal IgG increases the bactericidal efficiency of C...